Suppose that a TLC plate is spotted with samples 1.0 cm above the bottom of the

The compounds in the samples will leach into the pool of developing solvent resulting in poor resolution.

Explanation:

The aim of thin layer chromatography is the separation of a mixture. Usually, the stationary phase is spotted with the mixture. A mark is made with a pencil at the point to be spotted.

It is necessary to note that the solvent must be below the spot where the mixture is. This is because, if the solvent is above the spot of the mixture to be separated, the components of the mixture will dissolve away in the solvent resolution in poor separation. This is exactly what will happen when the sample is spotted at 1.0 cm above the bottom when the solvent is 1.2 cm above the bottom.

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