The compounds in the samples will leach into the pool of developing solvent resulting in poor resolution.
Explanation:
The aim of thin layer chromatography is the separation of a mixture. Usually, the stationary phase is spotted with the mixture. A mark is made with a pencil at the point to be spotted.
It is necessary to note that the solvent must be below the spot where the mixture is. This is because, if the solvent is above the spot of the mixture to be separated, the components of the mixture will dissolve away in the solvent resolution in poor separation. This is exactly what will happen when the sample is spotted at 1.0 cm above the bottom when the solvent is 1.2 cm above the bottom.
Energy is a set of physics measures. Popularly the term is most often used in the context of energy as a technology: energy resources, their consumption, development, depletion, and conservation. Biologically, bodies rely on food for energy in the same sense as industry relies on fuels to continue functioning. Since economic activities such as manufacturing and transportation can be energy intensive, energy efficiency, energy dependence, energy security and price are key concerns. Increased awareness of the effects of global warming has led to international debate and action for the reduction of greenhouse gases emissions, like many previous energy use patterns it is changing not due to depletion or supply constraints but due to problems with waste, extraction or geopolitical scenarios.
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