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The role of cytoskeletal fibers in cell division Replication of an animal cell requires the participation and coordination of microtubules, intermediate filaments, and microfilaments. Breakdown of the nuclear envelope : Before mitosis begins, the nuclear envelope is broken down by disassembling the lamins, a type of intermediate filament that supports the structure of the envelope. Mitotic spindle formation : Once the nuclear contents become continuous with the cytoplasm, the microtubules of the spindle grow out from the centrosomes at the two poles. The microtubules are captured by motors associated with the chromosomes. Formation of the nuclear envelope : Once the chromosomes have been separated, marking the end of anaphase, a nuclear envelope begins to form around each set of chromosomes through the assembly of new lamins and vesicles. Cytokinesis : Like a drawstring, a belt of microfilaments and their associated myosin motor proteins pull in the membrane between the two newly formed nuclei, constricting the cytoplasm and creating a furrow that eventually divides the cell.